Equine Neurological PCR Panel
The Equine Neurological PCR Panel is a diagnostic assay designed to detect the presence of key pathogens causing neurological disease in horses, including Equine Herpesvirus-1 (EHV-1), West Nile Virus (WNV), Eastern Equine Encephalitis Virus (EEE), and Sarcocystis neurona. This panel utilizes advanced real-time polymerase chain reaction (rtPCR) technology for the sensitive and specific identification of these pathogens in clinical samples such as cerebrospinal fluid (CSF), blood, or tissue.This panel includes
Equine Herpesvirus-1 (EHV-1): Known for causing Equine Herpesvirus Myeloencephalopathy (EHM), EHV-1 leads to CNS inflammation, resulting in ataxia, hind limb weakness, and urinary incontinence.
West Nile Virus (WNV): A mosquito-borne flavivirus, WNV causes encephalitis, with symptoms like fever, muscle fasciculations, and impaired coordination.
Eastern Equine Encephalitis (EEE): An arbovirus causing severe neurological disease, EEE often results in rapid onset of fever, depression, and seizures, with high mortality rates.
Sarcocystis neurona: The protozoan agent of Equine Protozoal Myeloencephalitis (EPM), S. neurona causes asymmetric muscle atrophy, weakness, and ataxia.
Test name
Equine Neurological PCR Panel
Test Code
E177
Section
Molecular Diagnostics
Species
Equine
Specimen
2 ml CSF
Collection Guidelines
Collect an adequate amount of CSF in a K3EDTA (purple top tube). Gently mix well after collection. Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 Days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Pusterla, N., et al. “Molecular Diagnostics for Equine Tick-Borne Diseases: Utility of Comprehensive rtPCR Panels.” Journal of Equine Veterinary Science, 2021.
- Hinchcliff, K.W., et al. “Advances in the Diagnosis of Equine Tick-Borne Pathogens Using Real-Time PCR.” Veterinary Clinics of North America: Equine Practice, 2022.
- Sellon, D.C., et al. “Detection and Management of Tick-Borne Pathogens in Horses: A Molecular Perspective.” Journal of Veterinary Diagnostic Investigation, 2023.
