Microbiota Dysbiosis Index
The Dysbiosis Index (DI) is a qPCR-based test that evaluates fecal microbiome imbalances in dogs and cats. It quantifies key bacterial taxa, assesses microbial diversity, and predicts bile acid metabolism, aiding in diagnosing chronic enteropathies and antibiotic-induced dysbiosis.
Test Code
5036
Section
Molecular Diagnostics
Species
Canine, Feline
Turnaround Time
Contact Vettto Team
Specimen
0.5 g feces in the given ethanol suspension
Collection Guidelines
- Collect up to 0.5 g of feces and mix it with 1 ml ethanol suspension in the provided container.
- Ensure the sample is well mixed and does not exceed 0.5 g.
- Preferably submit the container in a zip-locktype plastic bag.
Packaging Instructions
- Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label.
- Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Toresson, L., Spillmann, T., Pilla, R., Ludvigsson, U., Hellgren, J., Olmedal, G. and Suchodolski, J.S., 2023. Clinical effects of faecal microbiota transplantation as adjunctive therapy in dogs with chronic enteropathies—A retrospective case series of 41 Dogs. Veterinary sciences, 10 (4), p.271.
- Sung, C.H., Marsilio, S., Chow, B., Zornow, K.A., Slovak, J.E., Pilla, R., Lidbury, J.A., Steiner, J.M., Park, S.Y., Hong, M.P. and Hill, S.L., 2022. Dysbiosis index to evaluate the fecal microbiota in healthy cats and cats with chronic enteropathies. Journal of Feline Medicine and Surgery, 24(6), pp.e1-e12.
- AlShawaqfeh MK, Wajid B, Y. Minamoto, M. Markel, J.A. Lidbury, J.M. Steiner, E. Serpedin, and J.S. Suchodolski, A dysbiosis index to assess microbial changes in fecal samples of dogs with chronic inflammatory enteropathy. FEMS Microbiol Ecol (2017
- J. Chaitman, A.L. Ziese, R. Pilla, Y. Minamoto, A.B. Blake, B.C. Guard, A. Isaiah, J.A. Lidbury, J.M. Steiner, S. Unterer, and J.S. Suchodolski, Fecal microbial and metabolic profiles in dogs with acute diarrhea receiving either fecal microbiota transplantation or oral metronidazole. Front Vet Sci 7 (2020)
