Equine Diagnostics

Equine Parvovirus (PCR)

The Equine Parvovirus (EPV) PCR test is a sensitive molecular diagnostic tool designed to detect the DNA of equine parvovirus-hepatitis (EPV-H), a virus associated with Theiler’s disease, also known as serum hepatitis. EPV is thought to be transmitted via contaminated biologics such as equine serum or plasma products, though natural transmission routes remain under investigation. The disease can result in severe liver damage, presenting with symptoms such as lethargy, anorexia, jaundice, fever, and in some cases, acute hepatic failure. The PCR method amplifies EPV-specific genetic material, making it a highly accurate technique for detecting active infection in blood or liver tissue samples. This test is particularly useful for diagnosing Theiler’s disease in symptomatic horses and screening donor horses to ensure the safety of biologics used in veterinary medicine. Early detection enables timely intervention, preventing the spread of the virus and reducing the risk of outbreaks in equine populations.

Test name

Equine Parvovirus (PCR)

Test Code

E127

Section

Molecular diagnostics

Species

Equine

Specimen

2 ml EDTA whole blood 

Collection Guidelines

Collect an adequate amount of blood in a K3EDTA (purple top tube). Gently mix blood well after collection. Refrigerate sample at 4oC until shipment..

Turnaround time

14-21 Days

Packing Instructions

Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.

References

1. Tomlinson, J.E., et al. “Equine Parvovirus-Hepatitis: Diagnostic Approaches and Clinical Implications.” Journal of Veterinary Internal Medicine, 2021.
2. Pusterla, N., et al. “Application of PCR in Detecting Equine Parvovirus and Its Role in Theiler’s Disease.” Veterinary Clinics of North America: Equine Practice, 2022.
3. Divers, T.J., et al. “Advances in Equine Parvovirus Diagnosis: Insights into Theiler’s Disease.” Journal of Veterinary Diagnostic Investigation, 2023.