Anti-Müllerian Hormone (ELISA)
The Anti-Müllerian Hormone (AMH) ELISA test is a valuable tool for assessing reproductive status in dogs. AMH is a glycoprotein hormone produced by ovarian granulosa cells and testicular Sertoli cells, and its levels provide insights into gonadal function. This test is commonly used to confirm spay status, detect ovarian remnant syndrome in females, and differentiate between cryptorchid and castrated males. It is useful for the diagnosis of granulosa-theca cell tumors (GCTs).
Test name
Anti-Müllerian Hormone (ELISA)
Test Code
2003
Section
Clinical Pathology
Species
Canine and Feline
Specimen
2 ml Serum
Collection Guidelines
- Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
- Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as “serum.”
- Excessively hemolyzed samples are not suitable for this test.
- Unspun samples will not be accepted.
- Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Place, N.J., et al. “Use of Anti-Müllerian Hormone for Detection of Ovarian Remnant Syndrome and Spay Status in Dogs.” Journal of Veterinary Diagnostic Investigation, 2022.
- Romagnoli, S., & Concannon, P.W. “Reproductive Endocrinology and AMH Testing in Canine Practice.” Theriogenology, 2023.
- England, G.C.W., et al. “Clinical Applications of Anti-Müllerian Hormone in Veterinary Reproduction.” Veterinary Reproductive Science, 2021.
