Digital Copper Quantification
Quantification of copper in stained tissue slides involves assessing copper levels in formalin-fixed, paraffin-embedded (FFPE) liver tissue sections. This is achieved through Digital Copper Quantification (DCQ), a specialized technique that quantifies copper deposition in stained slides. DCQ is exclusively performed on liver biopsies submitted for liver consultations or as part of routine histopathology. The interpretation of results requires correlation with the distribution of copper in the tissue and its association with pathological features such as inflammation, degeneration, and fibrosis.
Test name
Digital Copper Quantification
Test Code
7002
Section
Histopathology
Species
Canine and Feline
Specimen
One of the following: fixed tissue in 10% formalin, paraffin embedded tissue, or 3 unstained formalin-fixed tissue sections on positively charged slides. Slide preparation using submitted tissue will incur a ₹1500 charge.
Collection Container
Tissue: Samples must be submitted in sealed, leak-proof, wide-mouth plastic containers. Glass containers are not acceptable and will incur an additional processing fee
Slides: Samples should be submitted in designated slide containers.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Center, S.A., et al. (2022). “Copper-Associated Hepatopathies in Veterinary Medicine: Diagnosis and Management.” Veterinary Clinics of North America: Small Animal Practice.
- Hoffmann, G., et al. (2023). “Quantitative Assessment of Hepatic Copper Using Digital Analysis in Veterinary Pathology.” Journal of Veterinary Diagnostic Investigation.
- Thornburg, L.P., et al. (2021). “Histopathologic and Molecular Techniques for Evaluating Copper Storage Disorders.” Journal of Veterinary Internal Medicine.