Tick-borne Pathogen Panel (rtPCR) – Equine
The Tick-Borne Pathogen Panel, performed using real-time polymerase chain reaction (rtPCR), is a comprehensive diagnostic test designed to detect the DNA of multiple tick-borne pathogens in equine samples. This panel screens for a broad range of bacterial and protozoal pathogens associated with significant diseases in horses, including:
Rickettsiales species: Anaplasma phagocytophilum (equine granulocytic anaplasmosis), Rickettsia rickettsii.
Borrelia species: Borrelia burgdorferi (Lyme disease),
Babesia species: Babesia caballi (equine piroplasmosis).
This rtPCR test is highly sensitive and specific, enabling the rapid identification of active infections, even in the early stages when clinical signs are subtle or nonspecific. Tick-borne diseases in horses can manifest as fever, anemia, lameness, lethargy, joint swelling, neurological symptoms, or systemic illness. Early and accurate detection is crucial for timely intervention and effective treatment, including targeted antimicrobial or antiprotozoal therapies.The panel is particularly useful in horses with suspected tick exposure, unexplained clinical signs, or those residing in endemic areas. Additionally, it supports the management of outbreaks and aids in reducing disease transmission within equine populations.
Test name
Tick-borne Pathogen Panel (rtPCR) – Equine
Test Code
E164
Section
Molecular Diagnostics
Species
Equine
Specimen
2 ml EDTA whole blood
Collection Guidelines
Collect an adequate amount of blood in a K3EDTA (purple top tube). Gently mix blood well after collection. Refrigerate sample at 4oC until shipment..
Turnaround time
14-21 Days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Pusterla, N., et al. “Molecular Diagnostics for Equine Tick-Borne Diseases: Utility of Comprehensive rtPCR Panels.” Journal of Equine Veterinary Science, 2021.
- Hinchcliff, K.W., et al. “Advances in the Diagnosis of Equine Tick-Borne Pathogens Using Real-Time PCR.” Veterinary Clinics of North America: Equine Practice, 2022.
- Sellon, D.C., et al. “Detection and Management of Tick-Borne Pathogens in Horses: A Molecular Perspective.” Journal of Veterinary Diagnostic Investigation, 2023.
