Listeria monocytogenes (PCR)
The Listeria monocytogenes (PCR) test uses polymerase chain reaction (PCR) technology to detect the DNA of Listeria monocytogenes, a bacterial pathogen responsible for listeriosis in animals and humans. This pathogen is known to cause serious health issues, including septicemia, encephalitis, and reproductive disorders such as abortions. Listeriosis is of significant zoonotic concern, particularly in food-producing animals, due to its ability to contaminate food supplies. The PCR test offers rapid, sensitive, and specific detection of L. monocytogenes, enabling early diagnosis and prompt intervention. This test is particularly valuable for identifying infections in symptomatic animals and for monitoring contamination in high-risk environments like farms and food production facilities.
Test name
Listeria monocytogenes (PCR)
Test Code
5021
Section
Molecular Diagnostics
Species
Canine and Feline
Specimen
2 ml EDTA whole blood
Collection Guidelines
Collect an adequate amount of blood in a K3 EDTA (purple top tube). Gently mix blood well after collection. Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Wiedmann, M., et al. (2023). “Listeriosis in Animals: Diagnostic Approaches and Public Health Implications.” Journal of Veterinary Diagnostic Investigation.
- Schoder, D., et al. (2022). “Advances in Molecular Detection of Listeria monocytogenes in Veterinary and Food Safety.” Veterinary Clinics of North America: Food Animal Practice.
- Oevermann, A., et al. (2021). “Pathogenesis and Diagnosis of Listeria monocytogenes Infections in Livestock.” Journal of Small Animal Practice.
