Francisella tularensis (rtPCR) – Rabbit
The Francisella tularensis (rtPCR) test is a molecular diagnostic tool designed to detect the genetic material of Francisella tularensis, the causative agent of tularemia, in rabbits. This test utilizes real-time polymerase chain reaction (rtPCR) technology for rapid, specific, and highly sensitive detection, even in early stages of infection or asymptomatic carriers.The rtPCR test provides several advantages over traditional diagnostic methods, including its ability to detect low bacterial loads and differentiate between Francisella tularensis subspecies Francisella tularensis is a zoonotic bacterial pathogen with significant implications for rabbit populations and public health. Infected rabbits may exhibit clinical signs such as fever, lethargy, lymphadenopathy, abscesses, or sudden death. However, subclinical infections can occur, making routine screening vital in high-risk settings, such as breeding facilities, laboratories, and wildlife rehabilitation centers.
Test name
Francisella tularensis (rtPCR) – Rabbit
Test Code
A111
Section
Molecular Diagnostics
Species
Rabbit
Specimen
1 ml EDTA whole blood
Collection Guidelines
Collect an adequate amount of blood in a K3 EDTA (purple top tube). Gently mix blood well after collection. Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 Days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Rohmer, L., et al. (2023). Genomic analysis of Francisella tularensis: Insights into pathogenicity and diagnostics. Frontiers in Microbiology, 14, 11876.
- Sjöstedt, A. (2022). Tularemia: Epidemiology, diagnosis, and treatment. Clinical Microbiology Reviews, 35(3), e00017-22.
- Dennis, D. T., et al. (2021). Advances in tularemia surveillance and diagnostics. Emerging Infectious Diseases, 27(5), 1048–1057.
- Keim, P., et al. (2020). Molecular diagnostics of Francisella tularensis: Challenges and opportunities. Journal of Clinical Microbiology, 58(8), e00842-20.
- Petersen, J. M., et al. (2020). Tularemia and the importance of wildlife reservoirs. Vector-Borne and Zoonotic Diseases, 20(4), 291–300.
